Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.
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Abstract Andrographis paniculata and Silybum marianum are well known medicinal plants. However, to prove their efficacy for clinical utilization more scientific data are needed. Therefore, in the present study, an attempt was made to investigate the anticancer potential of hydroalcoholic extracts of A.
The sulphorhodamine B SRB assay was used to assess growth inhibition of human tumor. Five human cancer cell lines i. The results obtained suggest that S. The combination of both the plants 1: The results obtained in the study indicate that A.
BioMed Research International
Cancer is the uncontrolled growth and division of cells and it leads to a mass of cells known as tumor. In the body normal cells complete their life andrographhis by following an orderly path of growth, division and death.
The process of programmed cell death is called apoptosis.
When apoptosis breaks down, it leads to cancer. Chemotherapy is a major clinical treatment used for the control of advanced stages of malignancies and metastasis. But many cancer patients seek alternative and complementary methods of treatment because of the serious side effects of chemotherapy and radiation therapy. Several herbs and panicuoata with diversified pharmacological properties have been shown to have potential to prevent human cancers as they possess antitumor substances which have the potential to cure cancer without causing toxicity.
Andrographis paniculata is an annual herb belongs to family Acanthaceae. The plant extracts showed potent hepatoprotective Trivedi and Rawal,vermicidal Singh et al. Silybum marianum is an annual or biennial erect herb belongs to the family Compositae. Different parts of this plant showed potent hepatoprotective Fraschini et al. The present study was undertaken to paniculaya the anticancer activity of two medicinal plant s Andrographis paniculata and Silybum marianum aerial part and andrograpgis combinational effects against five human cancer cell lines i.
The authenticated plants materials were collected from Natural Remedies Pvt. D and confirmed at Botany Department, Dr. Gour Central University, Sagar M.
The following drugs and chemicals were used: All chemicals used were of analytical grade. The filtrate was evaporated to dryness using a rotary evaporator yield was The extracts were then stored below ambient temperature for further studies. Phyto chemical analysis was performed to detect various compounds such as tannins, flavonoids, alkaloids, steroids etc.
Culturing of cell line: Maintained cultures were passage weekly and the culture medium was changed twice a week. The human tumor cytotoxicity was determined by the protocols established by NCI Monks et al.
The colorimetric assay estimated cell number indirectly by staining total cellular protein with the dye SRB. Single-cell suspensions were prepared by the treatment of cells with 0. The viable cells were counted using a Coulter counter and diluted with RPMI medium and final densities of x10 4 cells mL -1 were obtained.
After 24 h, the cells were treated with the extracts. For combinational study 1: The supernatant was then discarded and the plates were washed 5 times with deionised water and then dried. Bounded stain was solubilised with Tris buffer 10 mM and the optical densities were read on an automated spectrophotometric plate reader Molecular Devices, USA at a wavelength of nm, Optical density OD of SRB in each well is directly proportional to the cell number. Percentage inhibition was calculated by comparing the OD of control well with that of test sample:.
Phytochemical study showed the presence of flavonoids, tannins, carbohydrate, cardiac glycosides, terpenes and steroids in both the plant extracts.
Apart from this, alkaloids were present only in the extract of Andrographis paniculata while, saponins were present only in Silybum marianum extract Table 1. The preliminary examination was done by observing the confluency of the test as well as control samples which showed cell growth inhibition in the test samples Fig.
On the basis of measurement of OD, the anticancer study suggest that S.
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Andrographis paniculata extract was found most effective against Ovcar cell line Again, the combination ansrographis Medicinal plants maintain the health and vitality of individuals and cure various diseases, including cancer without causing toxicity.
These medicinal plant s possess good immunomodulatory and antioxidant properties, leading to anticancer activities. The antioxidant phytochemicals protect the cells from oxidative damage. Thus, consuming a diet rich in antioxidant plant material can provide health-protective effects. These natural products are supposed to minimize DNA damage by reacting with free radical s and actviity this way they can prevent cancer.
Phytochemicals, such as flavonols and flavonoids were investigated to determine chemoprevention activity against cancer Conese and Blasi, Phenols, polyphenols, flavonoids and their derivatives are ubiquitous in plants and have been found associated with the inhibition of atherosclerosis and cancer Cirla and Mann, Recent studies have reported antitumor effects of the flavonoids, quercetin, genistein and baicalein obtained from plant extracts Shoeb et al.
Similarly, alkaloids like schischkinnin and montamine have been isolated from the seeds of Centaurea schischkinii and Centaurea montana which showed anticancer property Yang and Wang, Flavonoids isolated from Plantago species were able to strongly inhibit the proliferation of human cancer cell lines Galvez et al.
In conclusions, the result supports the use of the plant as described in folk medicine, that the aerial plant parts can be used to treat cancer. Further studies are required to isolate the active constituents involved in the antioxidant and hepatoprotective activity of the plant. Prevention of common colds with Andrographis paniculata dried extract: A pilot double blind study. From natural products to drug discovery. Effect of silymarin on different acute inflammation models and on leukocyte migration.
Cytotoxic effect of Plantago spp. Protective effects of silymarin against photocarcinogenesis in a mouse skin model.
Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress
Hepatoprotective and immunological effects of antioxidant drugs. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. Immunostimulant agents from Andrographis paniculata. Montamine, a unique dimeric indole alkaloid, from the seeds of Centaurea Montana Asteraceae and its in vitro cytotoxic activity against the colon cancer cells. Anthelmintic potential of Andrographis paniculataCajanus cajan and Silybum marianum. Hepatoprotective and antioxidant property of Andrographis paniculata Nees in BHC induced liver damage in mice.
Experimental studies on prevention of atherosclerotic arterial stenosis and restenosis after angioplasty with Andrographis paniculata nees and fish oil. Journal of Pharmacology and Toxicology Volume 8 1: How to cite this article: Journal of Pharmacology and Toxicology, 8: Percentage inhibition was calculated by comparing the OD of control well with that of test sample: Phyto chemical analysis of hydroalcoholic extract of A.
Anticancer activity of hydroalcoholic extract of A. Anticancer activity of combinational effects of A.