We tested acute toxicity in worms Eisenia andrei following OCDE protocols ( ) and bioactivity tests in Artemia salina. The alkaloids fraction. Bioensayo de toxicidad en artemia salina. Cortés Montoya Vanessa Hernández Castañeda Miriam Najera Sánchez Gloria Guadalupe Ramírez Venegas María. Bioensayo de La Toxicidad Del Nitrato de Potasio en Reproductores de Artemia Salina en Condiciones de Laboratorio – Download as Word Doc .doc), PDF.
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However, in the present study, owing to logistic difficulties, the presence of cell content inside the alimentary canal of A. To the best of our knowledge, this is the first time the cytotoxicity and toxicity of phosphonium-based DESs were studied. For Artemia salina toxicity assay, the five most active were all from the 2nd and 3rd collections.
The dinoflagellate Alexandrium minutum and the haptophyte Prymnesium parvum are well known for their toxin production and negative effects in marine coastal environments. According to the aforementioned authors, excretion of toxic metabolites might be a general mechanism among toxic haptophytes when exposed to stress events, e. The effect of exposure time can be observed with LC 50 values at 24 and 48 h: Bioensayo de toxicidad en Artemia salina.
Quality control methods for medicinal plants materials. After equatorial dissection on biiensayo sea urchins Fig.
Antioxidant evaluations showed three of twelve were active aartemia two were considered moderately active, with a relationship dependently of concentration.
Artemia salina Research Papers –
The loss of PSP toxin production in a formerly toxic Alexandrium lusitanicum clone. References Publications referenced by this paper. Standard operational procedure, Creasel, Deinze, Belgium. Three replicates were prepared for each concentration.
There has been a recent increase in studies of the therapeutic characteristics plants in popular use in many places of the world. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. The Michaelis-Menten constant Km for starch of the Artemia nauplii amylase was estimated using Lineweaver-Burk plot to be 0.
The cytotoxicity of tested DESs was much higher than that of their individual components, indicating their toxicological behavior was different.
Six hundred specimens of C. Before being inoculated into the culture plate, the cell was transferred to a sterile culture medium drop and captured again, to eliminate possible contaminants.
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Anti-mitotic activity towards sea urchin embryos in extracts from the marine haptophycean Phaeocystis pouchetii Hariot Lagerheim collected along the coast of northern Norway.
Retos ante el tratado de libre comercio. The dinoflagellate Alexandrium minutum was toxic for A. Los extractos activos de D. The active extracts of D. The toxicity tests were performed in well polystyrene multiwell plates containing 1 mL of different cell concentrations Table 1. It was also found that there was toxic effect on the studied bacteria, indicating their potential application as anti-bacterial agents. Morphological, toxicological and genetic diversity.
On the other salins, larvae were considered non-well-developed when no four arms were formed Fig.
The most common is the mouse bioassay developed by the Japanese Ministry of Health and Welfare Yasumoto et al. Bioactive terpenoids from Caribbean marine algae of the genera Penicillus and Udotea Chlorophyta.
Test procedures and applications. PhD thesis, University of Vigo, pp. A marine diatom-derived aldehyde induces apoptosis in copepod saalina sea urchin embryos.
Química y biología del extracto etanólico del epicarpio de Crescentia cujete L. (totumo)
Artemia toxicity screening test for estuarine and marine waters. Effect of Caulerpa taxifolia secondary metabolites on the embryogenesis, bieonsayo development and metamorphosis of the sea urchin Paracentrotus lividus. In addition, the exposure time was increased from 24 to 48 hours.
Alexandrium minutum ; Prymnesium parvum ; Paracentrotus lividus ; Artemia franciscana ; Corophium multisetosum ; bioensayos de toxicidad. Toxic hazards dde man. A, Alexandrium minutum x and Prymnesium parvum y ; B, sea urchin Paracentrotus lividus ; Biosnsayo, amphipod Corophium multisetosum. Help Center Find new research papers eb This observation is in accordance with Garmendia et al.
Subphylum Crustacea Poisons substance larval development Larva hatching. Its life cycle begins with the hatching of dormant cysts that, once toxicidadd in salt water, start the development as nauplii larvae, which are the material for the bioassays. As a result, it cannot be concluded that the higher sensitivity of A. Nevertheless, our results place P. In the present study, bioassays with three different marine invertebrates with different levels of biological organization were applied as a detection tool to assess the toxic capacity of these marine microalgae species.
Moreover, obtaining gametes and in vitro fecundation do not involve difficulties, while the embryonic development is short, thus allowing viable larvae to be obtained in a short time Garmendia et al.
The arttemia was based on the exposure of the newly-fertilized eggs to different concentrations of cultured and filtered microalgae for 48 hours Table 1.
Thermal and pH stabilities ranged from 0oC to about 50oC and pH 6. L y Psidium guineense.