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Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.

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Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques. R f for nifedipine and carbamazepine were 0. The sampling was carried out under extremely subdued light and all tubes and syringes were wrapped in aluminum foil because of the photolability of nifedipine.

Later, blank serum samples were spiked with this solution, to obtain final concentrations of 0. Karimi, Talanta This method can be hpoc applied to drug adherence evaluation.

Book recommendation – Chromatography Forum

Apologies for any authoring errors And they are too old. Thank you guys, I’ll try to find them to buy, here in Brazil we intrroduccion not have many options, only a couple of books by local authors, but they treat the basics only. Trials2 ,S. Acta8 I’m sorry for my english, I’m Brazilian and I’m learning the language yet.


Chile, Chailapakul, Talanta 79, Complete resolution of the peaks with clear baseline separation was obtained of this way. No interference was observed. Therefore, the principal advantage of HPTLC is a fast analysis, with a high selectivity, accuracy, sensitivity nanograms in UV absorbance mode and picograms in fluorescence mode and precision.

Serum spiked with nifedipine standard to obtain concentrations of 0.

Introduccion a La HPLC Quattrocchi

No other method using HPTLC was quatrocchi in the literature, for the quantification of nifedipine in human serum neither in human plasma. In total there are 6 users online:: Best regards, Dmitriy A. The chromatographic conditions were arrived at after investigating several parameters, such as detection wavelength, ideal mobile phase and their proportions, internal standards, and optimal conditions for sample extraction.

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Application of the method The nifedipine concentration ranged between 0. Calcium-channel blockers, as nifedipine, are considered one of several preferred antihypertensive drugs for the initial management of hypertension in patients with a high risk of developing coronary artery disease, including those with diabetes mellitus.

Iowa State University Press, Chromatography Chromatography was carried out on HPTLC glass backed plates 10 x 10 cm and 20 x 10 cm precoated with silica gel Flayer thickness 0. Detection and quantification limits. The Merck veterinary manual.

Methods Standard solutions Nifedipine was dissolved with chloroform: The organic layer was transferred to another glass tube and the contents were evaporated to dryness at room temperature under a gentle stream of pure nitrogen. Inter-assay precision and inter-assay accuracy were calculated after repeated analysis in three different analytical runs.


Nifedipine also may be used in the management of hypertension in patients with low-renin hypertension as black patientscoexisting angina, peripheral vascular-disease, or isolated systolic hypertension, and in geriatric patients.

Introducción a la HPLC: aplicación y práctica – Oscar Alberto Quattrocchi – Google Books

There is a list of some recommended books on our web site: Actuators B Each experiment included the sample extraction step. Calibration curves Calibration curves were constructed using peak-area ratio for the nifedipine and the internal standard carbamazepine as a function of the concentration added.

The mobile phase consisted of chloroform: Book recommendation Basic questions from students; resources for projects and reports. Qusttrocchi method was applied to the determination of sulfamethazine in a veterinary commercial solution. Drug-free serum used for the validation of the method was obtained from healthy quattrochci.

The method was sensitive enough to detect a quantity as low as 0. These values were experimentally verified. Table 3 summarized all of validated values. The selectivity of the method was examined by preparing and analyzing checked by analyzing human serum samples spiked with nifedipine and the internal standard carbamazepine.